Run statistics ⓘ
| Measure | Value |
|---|---|
| Report name | ToulligQC_Demo_Data |
| Experiment group | Unknown |
| Sample ID | SmartLengthMinION_A2018 |
| Run ID | 39962aa0f19fcb4b023f9e88caa032ff9cfbfd26 |
| Run date | Wed Aug 01 13:42:54 UTC 2018 |
| Run duration | 47h59m58s |
| Flowcell ID | FAJ06689 |
| Flowcell product code | Unknown |
| Flowcell version | Unknown |
| Kit | Unknown |
| Sequencing kit | sqk-lsk108 |
| Barcode kits | Unknown |
| Selected speed (bps) | Unknown |
| Sample frequency (Hz) | 4000 |
| Yield | 7.62G |
| Read count | 2,932,487 |
| N50 (bp) | 3,230 |
| L50 | 2,063,112 |
Device and software ⓘ
| Measure | Value |
|---|---|
| Device type | minion |
| Device ID | MN17734 |
| Device hostname | dnacpc14 |
| Device OS | ubuntu 14.04 |
| Distribution version | Unknown |
| MinKNOW version | 1.11.5 |
| Basecaller name | guppy-basecalling |
| Basecaller version | 3.2.4+d9ed22f |
| Basecaller analysis | 1d_basecalling |
| Basecalling date | Wed Sep 04 07:56:30 UTC 2019 |
| Model file | template_r9.4.1_450bps_fast.jsn |
| Min qscore threshold | 7.00 |
| ToulligQC version | 2.4 |
| All reads | Pass reads | Fail reads | Pass barcoded reads | Fail barcoded reads | |
|---|---|---|---|---|---|
| count | 2,932,487 | 2,596,388 | 336,099 | 2,219,184 | 77,429 |
| percent | 100.00 | 88.54 | 11.46 | 75.68 | 2.64 |
| All reads | Pass reads | Fail reads | |
|---|---|---|---|
| count | 2,932,487 | 2,596,388 | 336,099 |
| mean | 2,598.87 | 2,668.29 | 2,062.59 |
| std | 1,434.31 | 1,426.18 | 1,382.75 |
| min | 7.00 | 100.00 | 7.00 |
| 25% | 1,559.00 | 1,646.00 | 951.00 |
| median | 2,366.00 | 2,442.00 | 1,898.00 |
| 75% | 3,488.00 | 3,557.00 | 2,885.00 |
| max | 44,486.00 | 44,486.00 | 22,606.00 |
| All reads | Pass reads | Fail reads | |
|---|---|---|---|
| count | 2,932,487 | 2,596,388 | 336,099 |
| mean | 9.76 | 10.34 | 5.25 |
| std | 2.03 | 1.24 | 1.02 |
| min | 2.16 | 7.00 | 2.16 |
| 25% | 8.96 | 9.59 | 4.57 |
| median | 10.36 | 10.56 | 5.23 |
| 75% | 11.17 | 11.26 | 6.07 |
| max | 16.44 | 16.44 | 6.00 |
| Barcode arrangement (%) | Read count | Base count | |
|---|---|---|---|
| barcode01 | 11.60% | 301,092 | 792,229,661 |
| barcode02 | 22.61% | 586,981 | 1,659,382,337 |
| barcode03 | 10.90% | 282,933 | 757,317,615 |
| barcode04 | 26.38% | 684,838 | 1,799,063,466 |
| barcode05 | 13.99% | 363,340 | 1,017,818,978 |
| unclassified | 4.99% | 129,647 | 302,504,364 |
| other barcodes | 9.53% | 247,557 | 599,597,980 |
| Barcode arrangement (%) | Read count | Base count | |
|---|---|---|---|
| barcode01 | 3.44% | 11,560 | 23,610,570 |
| barcode02 | 6.27% | 21,080 | 47,691,876 |
| barcode03 | 2.91% | 9,779 | 21,001,498 |
| barcode04 | 7.09% | 23,838 | 50,891,528 |
| barcode05 | 3.32% | 11,172 | 25,866,485 |
| unclassified | 74.30% | 249,723 | 506,695,118 |
| other barcodes | 2.66% | 8,947 | 17,477,494 |